string(4) "test" RAMAN SPECTROSCOPY AND COHERENT ANTI-STOKES RAMAN SCATTERING IMAGING: PROMISING TECHNIQUES FOR MONITORING SKELETAL CELLS AND BONE REGENERATION | International Conference On Higher Education Teaching
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Vol. 3 No. 2 (2025)
International Conference On Higher Education Teaching

Articles

RAMAN SPECTROSCOPY AND COHERENT ANTI-STOKES RAMAN SCATTERING IMAGING: PROMISING TECHNIQUES FOR MONITORING SKELETAL CELLS AND BONE REGENERATION

Published 2025-02-20

  • Malikov Murod Rasulovich

Abstract

Skeletal stem cells (SSCs) are a promising tool for cell-based therapies aimed at treating skeletal diseases and facilitating tissue repair. The ability to precisely modulate SSCs holds significant potential for regenerative medicine, offering the prospect of long-term stem cell engraftment and differentiation. Currently, SSC differentiation into stromal lineages such as bone, fat, and cartilage is typically assessed using methods that require cell fixation or lysis, which are often invasive or destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy provide a powerful, non-invasive alternative for studying biological systems in their natural state, preserving cellular integrity. This review explores the applications of Raman spectroscopy and CARS imaging in stem cell research, highlighting their potential for evaluating SSCs, skeletal tissues, and bone regeneration.

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